Service for bd accuri c6 systems and their related options will continue until april 15, 2021. As a result, most novice flow cytometry users find it so easy to use, that they can collect and analyze data in. The accuri c6 is a compact flow cytometer with a wide dynamic range of over 7 decades. Msds linked at the right cleaning solution, kr225 citric acid. Flowjo and your cytometer flowjo v10 documentation. Displaying accuri cflow data and using the cflow importer in fcs. Fcs files from flow cytometers with 18bit or higher digital signal processing, such as the bd accuri c6 flow cytometer system, often require adjustment of flowjos scaling factors to properly visualize the data. The bd accuri c6 plus personal flow cytometer is the newest generation of the bd accuri platform. The accuri c6 flow cytometer does not produce aerosols during normal operation. Life science applications of personal flow cytometry. Buy used bd accuri c6 at great prices from certified genetool.
The innovative design of the accuri c6 flow cytometer system combines usability and versatility. Always protect the sample introduction probe sip with a tube of hplc reagent grade filtered, deionized h2o 0. Technical note a guide to absolute counting on the accuri. Blue lion biotech flow cytometers bd accuri c6 flow. The first place to look for new and used flow cytometers.
It has a very easy set up and the software interface is easy to navigate, however the analysis using the cflow software is a little. Install and validate the bd accuri c6 flow cytometer with 6 and 8peak beads prior to installing the bd. The accuri system was engineered from the start to be compact, powerful and userfriendly, while also being affordable by most research laboratories. Bd biosciences accuri c6 personal flow cytometer software.
Cited in over 9,000 publications, the bd accuri c6 plus enables you to go beyond your current research potential. Even novice flow cytometry users find it so easy to learn and use that most can collect and analyze data in less than an hour. A guide to absolute counting on the accuri c6 flow cytometer. Accuri c6 with c sampler and computer, c6 software installed. Flow cytometry the bd accuri c6 flow cytometer was switched on and allowed to warm up for 15 minutes before analyses were begun. Bd accuri c6 flow cytometer quick set up guide please follow these directions for setting up your bd accuri c6 flow cytometer. Enhanced sensitivity, reliability, and capabilities bring flow cytometry even more within reach for new and experienced flow cytometry researchers. To fill the cleaning solution bottle green line, add 3 ml of 1030% citric acid and fill with di. Any one have good or bad experiences with the new bd accuri c6 plus. Bd accuri c6 flow cytometer instrument manual bd accuri c6 software will trigger the notification based on the volume of sheath run through the system or on the date since maintenance was last performed. The bd accuri c6 flow cytometer an affordable, fullfeatured, easytouse flow cytometer. The accuri c6 offers a budget friendly option for a bench top flow cytometer. Its intuitive software coupled with its myriad visualization modes make for an easytouse but comprehensive data acquisition and analysis instrument.
It features two light scatter detectors and four fluorescence detectors with optical filters optimized for the detection of fluorochromes such as fitc, pe, percp, and apc. Selected life science applications of flow cytometry. Using the bd accuri c6 cytometer for rapid and accurate. Bd accuri c6 software controls the bd accuri c6 flow cytometer system in order to acquire data, generate statistics, and analyze results. Performance validation addition, the flow rate used, relative to sample concentration, prior to running samples for absolute counting, validate system fluidics performance by running accuri volume validation beads qa120 according to product instructions.
Flow cytometry is used in research and clinical laboratories for many applications including chromosome analysis, cancer diagnosis, protein. The bd accuri c6 has the sensitivity, reliability, and capabilities needed to bring flow cytometry to the next level. Flow cytometry is a laser based technology used to count and sort cells, detect biomarkers and engineer proteins, by suspending cells in a stream of fluid and passing them by an electronic detection system. Flowjo can read and analyze fcs files from any cytometer. Bd biosciences bd accuri c6 plus community, manuals and. Description introducing the next generation bd accuri personal flow cytometer.
Find a flow cytometer for sale and auction at labx. The bd accuri c6 is a personal flow cytometer that brings flow cytometry within reach by being easy to use, simple to maintain, and affordable. The c6 uses two lasers, red and blue, to record events and has 4 fluorescence channels for a wide range of fluorescence detection. Used flow cytometers bd facsaria, facscanto and accuri c6. Evaluation of an easy and affordable flow cytometer for. Bd accuri c6 plus flow cytometer with its compact 11 x 14. This small but powerful accuri c6 comes computer loaded with bd accuri c6 plus software. Breakthrough with the bd accuri c6 plus cell analyzer. Flow cytometers life science classifieds new and used. Bd accuri c6 flow cytometry system available for sale. Software options and instrument controls are clearly visible from the softwares tabbed interface.
Flow cytometry within reach the analytical power and versatility of todays laserbased flow cytometry systems have unlocked the mysteries of cell biology and empowered entirely new fields of research. Ive recently started doing flow cytometry bd accuri c6 in a core facility at my university, but my lab doesnt have any softtware to analyze the data. Current extended warranties will not be affected and will be supported until they expire. Bd accuri c6 plus software files can be exported in fcs 3. Bd has discontinued selling the bd accuri c6 flow cytometer as of december 31, 2016. Each cytometer will scale your data slightly different during acquisition. Refer to bd accuri c6 software user guide, chapter 1.
The bd accuri c6 plus flow cytometer system the instrument weighs just. Skip to navigation skip to content search, browse, and buy used lab equipment from biosurplus inc. It is a small but powerful accuri c6 comes computer loaded with bd accuri c6 plus software. As you probably know, fcs flow cytometry standard files are created by flow cytometers. Bd accuri c6 software will display this notification based on. Bd accuri c6 with single tube loader protocol this protocol contains instructions for setting up an accuri c6 with tube loader. Expanding fluorescence detection options with the accuri. This portable and compact system holds the analytical power of a cytometer twice its size. Does anyone have any recommendations for free software. Take a leap in flow cytometry with the bd accuri c6 plus. Components of a flow cytometer fluidic system optical system excitation and detection electronic system and software. The bd accuri c6 flow cytometer system includes bd accuri c6 software for controlling the instrument, generating statistics, and analyzing results.
Buy from accuri, or make your own citric acid solution. Bd accuri c6 cytometer manual getting ready ensure that the fluid levels in the sheath, cleaning, and decontamination bottles are sufficient. The bd accuri c6 flow cytometer along with the bd cytometric bead array reagent sets, provides researchers a. Performance validation of the bd accuri c6 was then done. The following conditions may indicate a problem with the cytometer or the validation beads.
Facsdiva v6 software overview course bd biosciences accuri c6 software user guide. Can anyone suggest good free flow cytometry software that can read. Accuri c6 software automatically displays the percentage of cells within the region. The bd accuri c6 plus offers simplified workflows and readytouse templates for performing a wide range of applications in flow cytometry. However, the accompanying software isnt the best, but it is free to use and does the job. Instrument includes a c sampler, computer, software, validation report and warranty. Flow cytometer ucsb shared instrumentation network. Bd accuri c6 software has an intuitive user interface that was developed based on hundreds of hours observing researchers using flow cytometers. Bd accuri c6 plus software features an intuitive user interface.
Bd accuri c6 flow cytometer from bd biosciences selectscience. The analytical power and versatility of todays laserbased flow cytometry systems have unlocked the mysteries of cell biology and empowered entirely new fields of. The accuri c6 flow cytometer is an increasingly popular flow cytometer. Waste and sheath fluid bottles were checked during this time, and emptied or refilled as required. If you have questions or require further clarification, see the bd accuri c6 flow cytometer instrument manual or contact accuri technical support. Service for bd accuri c6 systems and their related options will continue until december 31 st, 2021.
The bd accuri c6 flow cytometer reports arithmetic means. Bd biosciences accuri c6 personal flow cytometer overview. As a result, most novice flow cytometry users find it so easy to use, that they can collect and analyze data in less than an hour. Each time an activation key is used to install a new bd component, the dialog accuri c6 software box is updated to reflect the. It is a personal flow cytometer that brings cell analysis within reach by easy to use, simple to maintain, and affordable. Within 5 minutes of completing the water run, place a.